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MB Sample ID: SA083111
Local Sample ID: | 16-Synechocystis_6803-cell-4-1 |
Subject ID: | SU001262 |
Subject Type: | Bacteria |
Subject Species: | Synechocystis sp. PCC 6803 |
Taxonomy ID: | 1148 |
Genotype Strain: | NCBI:txid1148 |
Cell Biosource Or Supplier: | ATCC |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001991 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Thermo ISQ |
Column | Trace 1310 GC |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Thermo ISQ |
Ion Mode | POSITIVE |
Units | spectral abundance per cell |
Chromatography:
Chromatography ID: | CH001439 |
Chromatography Summary: | For non-targeted GC-MS experiments, metabolites were detected using a Trace 1310 GC coupled to a Thermo ISQ mass spectrometer. Samples (1 µL) were injected at a 10:1 split ratio to a 30 m TG-5MS column (Thermo Scientific, 0.25 mm i.d., 0.25 μm film thickness) with a 1.2 mL/min helium gas flow rate. GC inlet was held at 285°C. The oven program started at 140°C for 1 min, followed by a ramp of 15°C/min to 330°C, and 5 min hold. Masses between 50-650 m/z were scanned at 5 scans/sec under electron impact ionization. Transfer line and ion source were held at 300 and 260°C, respectively. Pooled QC samples were injected after every 6 actual samples. |
Instrument Name: | Thermo ISQ |
Column Name: | Trace 1310 GC |
Chromatography Type: | GC |