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MB Sample ID: SA086272
Local Sample ID: | QC-5 |
Subject ID: | SU001288 |
Subject Type: | Invertebrate |
Subject Species: | Bombus impatiens |
Taxonomy ID: | 132113 |
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Combined analysis:
Analysis ID | AN002034 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity I-Class |
Column | Waters Acquity CSH Phenyl-Hexyl (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Waters Synapt-G2-Si |
Ion Mode | POSITIVE |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH001474 |
Chromatography Summary: | We used a Synapt G2-Si quadrupole time-of-flight mass spectrometer (Waters, Milford, MA) coupled to an I-class UPLC system (Waters) for LC-MS analyses in the UC Riverside Metabolomics Core Facility. We carried out separations on a CSH phenyl-hexyl column (2.1 x 100 mm, 1.7 µM) (Waters, Milford, MA), with the following mobile phases: A. Water with 0.1% formic acid and B. Acetonitrile with 0.1% formic acid at a flow rate of 250 µL/min at 40° C. We injected 2 µL of sample extract, and the gradient was as follows: 0 min, 1% B; 1 min, 1% B; 8 min, 40% B; 24 min, 100% B; 26.5 min, 100% B; 27 min, 1% B. |
Methods Filename: | jroth002_LC_method.docx |
Instrument Name: | Waters Acquity I-Class |
Column Name: | Waters Acquity CSH Phenyl-Hexyl (100 x 2.1mm,1.7um) |
Column Temperature: | 40 |
Flow Gradient: | 0 min, 1% B; 1 min, 1% B; 8 min, 40% B; 24 min, 100% B; 26.5 min, 100% B; 27 min, 1% B. |
Flow Rate: | 250 µL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |