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MB Sample ID: SA086276
| Local Sample ID: | BBSe-1 |
| Subject ID: | SU001288 |
| Subject Type: | Invertebrate |
| Subject Species: | Bombus impatiens |
| Taxonomy ID: | 132113 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002034 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity I-Class |
| Column | Waters Acquity CSH Phenyl-Hexyl (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt G2 Si QTOF |
| Ion Mode | POSITIVE |
| Units | Peak Area |
Chromatography:
| Chromatography ID: | CH001474 |
| Chromatography Summary: | We used a Synapt G2-Si quadrupole time-of-flight mass spectrometer (Waters, Milford, MA) coupled to an I-class UPLC system (Waters) for LC-MS analyses in the UC Riverside Metabolomics Core Facility. We carried out separations on a CSH phenyl-hexyl column (2.1 x 100 mm, 1.7 µM) (Waters, Milford, MA), with the following mobile phases: A. Water with 0.1% formic acid and B. Acetonitrile with 0.1% formic acid at a flow rate of 250 µL/min at 40° C. We injected 2 µL of sample extract, and the gradient was as follows: 0 min, 1% B; 1 min, 1% B; 8 min, 40% B; 24 min, 100% B; 26.5 min, 100% B; 27 min, 1% B. |
| Methods Filename: | jroth002_LC_method.docx |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters Acquity CSH Phenyl-Hexyl (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0 min, 1% B; 1 min, 1% B; 8 min, 40% B; 24 min, 100% B; 26.5 min, 100% B; 27 min, 1% B. |
| Flow Rate: | 250 µL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
