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MB Sample ID: SA091302
Local Sample ID: | McN-Mat-20180411-03 |
Subject ID: | SU001324 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | DBA/2J-mdx |
Age Or Age Range: | 6 months |
Gender: | Male |
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Combined analysis:
Analysis ID | AN002085 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Ultimate3000 |
Column | Waters XBridge Amide (100 x 4.6mm,3.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q-Exactive |
Ion Mode | UNSPECIFIED |
Units | peak area values |
Chromatography:
Chromatography ID: | CH001522 |
Chromatography Summary: | 200ul of 50% Acetonitrile were added to the tube for reconstitution following by overtaxing for 1 min. Samples solution were then centrifuged for 15 min @ 20,000g, 4°C. Supernatant was collected for LC-MS analysis for Hydrophilic Metabolites Profiling as follows. Samples were analyzed by High-Performance Liquid Chromatography and High-Resolution Mass Spectrometry and Tandem Mass Spectrometry (HPLC-MS/MS). Specifically, system consisted of a Thermo Q-Exactive in line with an electrospray source and an Ultimate3000 (Thermo) series HPLC consisting of a binary pump, degasser, and auto-sampler outfitted with a Xbridge Amide column (Waters; dimensions of 4.6 mm × 100 mm and a 3.5 µm particle size). The mobile phase A contained 95% (vol/vol) water, 5% (vol/vol) acetonitrile, 20 mM ammonium hydroxide, 20 mM ammonium acetate, pH = 9.0; B was 100% Acetonitrile. The gradient was as following: 0 min, 15% A; 2.5 min, 30% A; 7 min, 43% A; 16 min, 62% A; 16.1-18 min, 75% A; 18-25 min, 15% A with a flow rate of 400 μL/min. |
Instrument Name: | Thermo Ultimate3000 |
Column Name: | Waters XBridge Amide (100 x 4.6mm,3.5um) |
Flow Gradient: | 0 min, 15% A; 2.5 min, 30% A; 7 min, 43% A; 16 min, 62% A; 16.1-18 min, 75% A; 18-25 min, 15% A |
Flow Rate: | 400 µL/min |
Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium hydroxide; 20 mM ammonium acetate, pH 9.0 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |