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MB Sample ID: SA092414
Local Sample ID: | 9pre |
Subject ID: | SU001339 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Combined analysis:
Analysis ID | AN002111 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu 20AD |
Column | ACQUITY UPLC BEH C18 (1.7 µm, 2.1 mm X 5 mm);Agilent ZORBAX StableBond C18 UHPLC guard (4.6 mm, 1.8 µm) |
MS Type | ESI |
MS instrument type | Other |
MS instrument name | Shimadzu LCMS-IT-TOF |
Ion Mode | POSITIVE |
Units | Area |
Chromatography:
Chromatography ID: | CH001541 |
Chromatography Summary: | Two solvents were used: (A) deionized water with 0,1% formic acid, and (B) MeCN. After a 5 min hold at 5.0% solvent B, elution was performed according to the following conditions: from 5.0% B to 59% B in 30 min; to 95% B in 7 min; keeping constant for 10 min, to 5% B in 3 min, followed by 5 min of equilibration on the initial conditions. The flow rate was 0.3 mL•min-1. The injection volume was 5 μL. |
Chromatography Comments: | Acquity UPLC BEH; 1.7 μm 2.1 × 5 mm UHPLC Guard 3PK Zorbax SB-C18 1.8 μm guard column |
Instrument Name: | Shimadzu 20AD |
Column Name: | ACQUITY UPLC BEH C18 (1.7 µm, 2.1 mm X 5 mm);Agilent ZORBAX StableBond C18 UHPLC guard (4.6 mm, 1.8 µm) |
Column Pressure: | 300-500 bar |
Column Temperature: | 30 |
Flow Gradient: | non-linear |
Flow Rate: | 0.3 ml/min |
Injection Temperature: | 5 |
Internal Standard: | papaverine |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |