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MB Sample ID: SA093097
Local Sample ID: | MMV688776_2 |
Subject ID: | SU001346 |
Subject Type: | Cultured cells |
Subject Species: | Trypanosoma brucei brucei |
Taxonomy ID: | 5702 |
Genotype Strain: | 427 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002115 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 RS |
Column | SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | Peak height |
Chromatography:
Chromatography ID: | CH001548 |
Chromatography Summary: | Metabolite extracts were analysed using hydrophilic interaction (HILIC) liquid chromatography (LC) and high resolution mass spectrometry on an Orbitrap system. |
Chromatography Comments: | Dionex RSLC3000 UHPLC (Thermo). Column additional info: Merck Sequant ZIC-pHILIC 5 µm polymer metal-free(150 x 4.6 mm) |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
Column Temperature: | 25°C |
Flow Gradient: | linear gradient -time, %B as follows: 0min- 80%, 15min- 50%, 18min- 5%, 21min- 5%, 24min- 80%, 32min- 80%. |
Flow Rate: | 300 μL/min |
Internal Standard: | internal standards (CHAPS, CAPS, PIPES and TRIS; all at 1 µM) |
Sample Injection: | 10 μL |
Solvent A: | 100% water; 20 mM ammonium carbonate |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |