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MB Sample ID: SA093758
Local Sample ID: | 24HKR4 |
Subject ID: | SU001363 |
Subject Type: | Plant |
Subject Species: | Triticum durum |
Taxonomy ID: | 4567 |
Genotype Strain: | cv. Opera |
Age Or Age Range: | 10 days |
Gender: | Not applicable |
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Combined analysis:
Analysis ID | AN002145 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Trace GC 1310, Thermo Fisher Scientific |
Column | TG-5MS |
MS Type | EI |
MS instrument type | GC-ITQ |
MS instrument name | ISQ LT, Thermo Fisher Scientific |
Ion Mode | POSITIVE |
Units | Relative Abundance |
Chromatography:
Chromatography ID: | CH001570 |
Chromatography Summary: | The derivatized extracts were injected into a TG-5MS capillary column (30 m x 0.25 mm x 0.25 µm) (Thermo Fisher Scientific, Waltham, MA, USA) using a gas chromatograph apparatus (Trace GC 1310, Thermo Fisher Scientific, Waltham, MA, USA) equipped with a single quadrupole mass spectrometer (ISQ LT, Thermo Fisher Scientific, Waltham, Massachusetts, US). Injector and source were set at 250°C and 260°C temperature, respectively. One µl of sample was injected in splitless mode with a helium flow of 1 ml/min using the following programmed temperature: isothermal 5 min at 70 °C followed by a 5°C/ min ramp to 350 °C and a final 5 min heating at 330°C. |
Methods ID: | NA |
Instrument Name: | Trace GC 1310, Thermo Fisher Scientific |
Column Name: | TG-5MS |
Column Temperature: | programmed temperature: isothermal 5 min at 70 °C followed by a 5°C/ min ramp to 350 °C and a final 5 min heating at 330°C |
Flow Rate: | 1 ml/min |
Injection Temperature: | 250 |
Internal Standard: | Adonitol (0.0002mg/ml of pure water) 60 μL per sample |
Retention Index: | Alkanes mixture C10-C40 |
Retention Time: | Alkanes mixture C10-C40 |
Oven Temperature: | programmed temperature: isothermal 5 min at 70 °C followed by a 5°C/ min ramp to 350 °C and a final 5 min heating at 330°C |
Transferline Temperature: | 250 |
Sample Syringe Size: | 10 μL |
Randomization Order: | YES |
Chromatography Type: | GC |