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MB Sample ID: SA094665
Local Sample ID: | ncV3 |
Subject ID: | SU001385 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague Dawley |
Age Or Age Range: | 70 days old |
Gender: | Male |
Animal Animal Supplier: | Harlan |
Animal Housing: | polycarbonate-free caging |
Animal Light Cycle: | 14-hr light and 10-hr dark |
Animal Feed: | Phytoestrogen Reduced II 18-5, Ziegler Bros, Inc |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002182 | AN002183 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Shimadzu Nexera-x2 | Shimadzu Nexera-x2 |
Column | 1.8 μm particle 50 × 2.1 mm | 1.8 μm particle 50 × 2.1 mm |
MS Type | ESI | ESI |
MS instrument type | Triple TOF | Triple TOF |
MS instrument name | ABI Sciex 5600 TripleTOF | ABI Sciex 5600 TripleTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak intensity | peak intensity |
Chromatography:
Chromatography ID: | CH001597 |
Instrument Name: | Shimadzu Nexera-x2 |
Column Name: | 1.8 μm particle 50 × 2.1 mm |
Column Temperature: | 55 |
Flow Gradient: | linear gradient over a 20 min total run time, with 60% solvent A and 40% solvent B gradient in the first 10 minutes, then the gradient was ramped in a linear fashion to 100% solvent B which was maintained for 7 minutes. After that the system was switched back to 60% solvent B and 40% solvent A for 3 minutes. |
Flow Rate: | 0.4 mL/min |
Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001598 |
Instrument Name: | Shimadzu Nexera-x2 |
Column Name: | 1.8 μm particle 50 × 2.1 mm |
Column Temperature: | 55 |
Flow Gradient: | linear gradient over a 20 min total run time, with 60% solvent A and 40% solvent B gradient in the first 10 minutes, then the gradient was ramped in a linear fashion to 100% solvent B which was maintained for 7 minutes. After that the system was switched back to 60% solvent B and 40% solvent A for 3 minutes. |
Flow Rate: | 0.4 mL/min |
Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |