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MB Sample ID: SA094734
Local Sample ID: | CI_27 |
Subject ID: | SU001388 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Cell Primary Immortalized: | Islets isolated from pancreata of adult C57BL/J6 male mice |
Cell Counts: | 100 islets |
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Combined analysis:
Analysis ID | AN002188 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH001603 |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
Column Pressure: | 6000-10000 psi |
Column Temperature: | 50 |
Flow Rate: | 0.4 ml/min |
Injection Temperature: | 8 |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Analytical Time: | 22 min |
Capillary Voltage: | 3.75 KV |
Weak Wash Solvent Name: | 10:90 Methanol:Water with 0.1% FA solution |
Strong Wash Solvent Name: | 75:25 2-Propanol: Water with 0.1% FA solution |
Randomization Order: | Yes |
Chromatography Type: | Reversed phase |