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MB Sample ID: SA097892
Local Sample ID: | AR414_Low_T_GFP_5pt2 |
Subject ID: | SU001422 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl/6JMarp |
Age Or Age Range: | 7 to 12 weeks |
Gender: | Male |
Animal Animal Supplier: | Monash University Animal Research Platform, Clayton, AUS |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002242 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 RS |
Column | SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH001648 |
Chromatography Summary: | Chromatography utilized a ZIC-pHILIC column (column temperature 25 °C) with a gradient elution of 20 mM ammonium carbonate (A) and acetonitrile (B) (linear gradient time-%B as follows: 0 min-80%, 15 min-50%, 18 min-5%, 21 min-5%, 24 min-80%, 32 min-80%) on a Dionex RSLC3000 UHPLC (Thermo). The flow rate was maintained at 300 μL/min. Samples were kept at 4 °C in the autosampler and 10 μL injected for analysis. |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
Column Temperature: | 25 |
Flow Gradient: | linear gradient time-%B as follows: 0 min-80%, 15 min-50%, 18 min-5%, 21 min-5%, 24 min-80%, 32 min-80% |
Flow Rate: | 300 µL/min |
Solvent A: | 100% water; 20 mM ammonium carbonate |
Solvent B: | 100% water; acetonitrile |
Chromatography Type: | HILIC |