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MB Sample ID: SA099584

Local Sample ID:KAS5309.fullMS.pos
Subject ID:SU001444
Subject Type:Fungi
Subject Species:Alternaria sp.
Taxonomy ID:1715220

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Combined analysis:

Analysis ID AN002286 AN002287
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Agilent ZORBAX RRHD Eclipse Plus C18 (50 x 2.1 mm,1.8 µm) Agilent ZORBAX RRHD Eclipse Plus C18 (50 x 2.1 mm,1.8 µm)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Integrated peak area Integrated peak area

Chromatography:

Chromatography ID:CH001680
Chromatography Summary:LC-MS conditions for negative and positive ionization modes
Instrument Name:Agilent 1290 Infinity
Column Name:Agilent ZORBAX RRHD Eclipse Plus C18 (50 x 2.1 mm,1.8 µm)
Column Temperature:35
Flow Gradient:0%B held for 0.5 minutes, increased to 100%B over 3.5 minutes. Hold B at 100% for 1.5 minutes, and then return to 0%B over 0.5 minutes
Flow Rate:0.3 mL/min
Sample Injection:2 µL
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Capillary Voltage:(-) 3.7 kV; (+) 3.9 kV
Chromatography Type:Reversed phase
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