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MB Sample ID: SA114153
| Local Sample ID: | POTR_03_sAT |
| Subject ID: | SU001480 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 11-20 years |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002349 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Thermo Trace 1310 |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive GC Orbitrap GC-MS/MS |
| Ion Mode | POSITIVE |
| Units | pg/g |
Chromatography:
| Chromatography ID: | CH001721 |
| Chromatography Summary: | Tissue extracts were analyzed using a Thermo Scientific 1310 gas chromatograph connected to a Q Exactive GC Orbitrap GC-MS/MS ultra-high-resolution mass spectrometer and Triplus RSH autosampler. A 2 µL aliquot of extract was injected into an inlet maintained at 250ºC in pulsed split-less mode. The analytes were separated on an Agilent DB-5MSUI capillary column (30m length × 0.25mm inner diameter × 0.25µm film thickness) using high purity helium (99.999% purity) as the carrier gas at a constant flow rate of 1 mL/min. The oven temperature program consisted of an initial temperature of 100ºC for 1 min, increased to 180ºC at 25ºC/min; followed by a temperature ramp to 215ºC at 5ºC/min, and finally increased to 300ºC at 25ºC/min and held for 10 min, resulting in a total run time of 26.6 min. |
| Instrument Name: | Thermo Trace 1310 |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Flow Rate: | 1 mL/min |
| Injection Temperature: | 250C |
| Internal Standard: | [13C6]-Anthracene, [13C12]-PCB28, [DIETHYL-D10]-Chlorpyrifos, [13C12]-PCB101, [13C12]-4,4'-DDE, [13C12]-PCB153, [13C12]-PCB180, [13C12]-PBDE47, [13C10]-Mirex, [13C6]-cis-Permethrin, [13C12]-PBDE99 and [13C12]-PBB153 |
| Sample Injection: | 2 uL |
| Analytical Time: | 26.6 |
| Oven Temperature: | The oven temperature program consisted of an initial temperature of 100ºC for 1 min, increased to 180ºC at 25ºC/min; followed by a temperature ramp to 215ºC at 5ºC/min, and finally increased to 300ºC at 25ºC/min and held for 10 min |
| Transferline Temperature: | 280 |
| Sample Syringe Size: | 10uL |
| Chromatography Type: | GC |
