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MB Sample ID: SA116251
Local Sample ID: | F_4m_1a |
Subject ID: | SU001489 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 4 months & 20 months |
Gender: | Male and female |
Animal Animal Supplier: | Jackson Laboratories |
Animal Housing: | SPF animal facility at USC |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002365 | AN002366 | AN002367 | AN002368 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) | Agilent Zorbax SBaq (50 x 2.1mm,1.7um) | Agilent Zorbax SBaq (50 x 2.1mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | MS count | MS count | MS count | MS count |
Chromatography:
Chromatography ID: | CH001735 |
Chromatography Summary: | HILIC experiments were performed using a ZIC-HILIC column 2.1x100 mm, 3.5μm, 200Å (Merck Millipore) and mobile phase solvents consisting of 10mM ammonium acetate in 50/50 acetonitrile/water (A) and 10 mM ammonium acetate in 95/5 acetonitrile/water (B).(Contrepois et al., 2015) |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
Column Temperature: | 40 |
Flow Rate: | 0.5 ml/min |
Solvent A: | 95% acetonitrile/5% water; 10 mM ammonium acetate |
Solvent B: | 95% acetonitrile/5% water; 10 mM ammonium acetate |
Chromatography Type: | HILIC |
Chromatography ID: | CH001736 |
Chromatography Summary: | RPLC experiments were performed using a Zorbax SBaq column 2.1 x 50 mm, 1.7 μm, 100Å (Agilent Technologies) and mobile phase solvents consisting of 0.06% acetic acid in water (A) and 0.06% acetic acid in methanol (B). (Contrepois et al., 2015) |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | Agilent Zorbax SBaq (50 x 2.1mm,1.7um) |
Column Temperature: | 60 |
Flow Rate: | 0.6 ml/min |
Solvent A: | 100% water; 0.06% acetic acid |
Solvent B: | 100% methanol; 0.06% acetic acid |
Chromatography Type: | Reversed phase |