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MB Sample ID: SA151896
| Local Sample ID: | P16_t0 |
| Subject ID: | SU001733 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002703 | AN002704 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 6550 | Agilent 6550 |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Intensity | intensity |
Chromatography:
| Chromatography ID: | CH001995 |
| Chromatography Summary: | The chromatographic separation was performed by using an UPLC BEH C18 (100 x 2.1 mm, 1.7 µm, Waters, Wexford, Ireland) column from Waters (Wexford, Ireland). Autosampler and column temperatures were set to 4°C and 40°C, respectively, and the injection volume was 5 µL. Mobile phase A and B consisted of H20 and acetonitrile, respectively, both containing 0.1% formic acid. The gradient elution lasted 14 minutes at a flow rate of 400 µl min-1. The mobile phase A was maintained at 98% for 1 min, and then decreased until 75% in 2 minutes, 50% in 3 minutes and 5% in 3 more minutes. 95% of mobile phase B was held for 3 min and then, a 0.55 min gradient was used to return to the initial conditions, which were held for 2.5 min to full column recovery. |
| Instrument Name: | Agilent 6550 |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | The mobile phase A was maintained at 98% for 1 min, and then decreased until 75% in 2 minutes, 50% in 3 minutes and 5% in 3 more minutes. 95% of mobile phase B was held for 3 min and then, a 0.55 min gradient was used to return to the initial conditions, which were held for 2.5 min to full column recovery. |
| Flow Rate: | 400 ul/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
