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MB Sample ID: SA152080
| Local Sample ID: | DNA_Adducts_Blank_1 |
| Subject ID: | SU001738 |
| Subject Type: | Synthetic sample |
| Subject Species: | - |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002712 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS |
| Column | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Ion trap |
| MS instrument name | Thermo Fusion Tribrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | Abundance |
Chromatography:
| Chromatography ID: | CH002001 |
| Chromatography Summary: | All analyses were conducted using identical chromatographic conditions and MS instrument settings, unless otherwise described. An UltiMate™ 3000 RSLCnano HPLC system (Thermo Scientific, Waltham, MA) was interfaced to an Orbitrap Fusion™ Tribrid™ MS (Thermo Fisher Scientific, San Jose, CA). One microliter of the authentic DNA standard mixture and five microliters of E. Coli DNA extracts were injected onto the analytical platform equipped with a 5 µL injection loop. Solvent blanks were analyzed before and after acquisition to assess contamination and sample carryover between injections. Chromatographic separation was performed using a custom-packed capillary column (75 µm ID, 20 cm length, 10 µm orifice) using a commercially available fused-silica emitter (New Objective, Woburn MA) containing Luna C18 (Phenomenex Corp. Torrance, CA) stationary phase (5 µm, 120 Å). The LC solvents were (A) 0.05% HCO2H in H2O and (B) CH3CN solutions. The flow rate was 1000 nL/min for 5.5 min at 2% B, then decreased to 300 nL/min with a 25 min linear gradient from 2 to 50% B, an increase to 98% B in 1 min, with a 4 min hold and a 5 min equilibration at 1000 nL/min to the starting conditions. The injection valve was switched at 5.5 min to remove the sample loop from the flow path during the gradient. A Nanospray Flex ion source (Thermo Fisher Scientific) was used with a source voltage of 2.2 kV and capillary temperature of 300°C. The S-Lens RF level setting was 60%. |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) |
| Flow Gradient: | The flow rate was 1000 nL/min for 5.5 min at 2% B, then decreased to 300 nL/min with a 25 min linear gradient from 2 to 50% B, an increase to 98% B in 1 min, with a 4 min hold and a 5 min equilibration at 1000 nL/min to the starting conditions. |
| Flow Rate: | 1ml/min-0.3ml/min |
| Solvent A: | 100% water; 0.05% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
