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MB Sample ID: SA152097
Local Sample ID: | mv08618_45.mzXML |
Subject ID: | SU001739 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Pooled |
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Combined analysis:
Analysis ID | AN002713 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 6550 |
Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
MS Type | EI |
MS instrument type | QTOF |
MS instrument name | Agilent 6550 QTOF |
Ion Mode | POSITIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH002002 |
Chromatography Summary: | The metabolomic analysis was carried out using an Ultra-Performance Liquid Chromatography (UPLC) system coupled to an iFunnel Q-ToF (quadrupole-time-of-flight) Agilent 6550 mass spectrometer (Agilent Technologies, CA, USA) using an UPLC BEH C18 (100 x 2.1 mm, 1.7 µm, Waters, Wexford, Ireland) column from Waters (Wexford, Ireland). Autosampler and column temperatures were set to 4 °C and 40 °C, respectively, and the injection volume was 5 µl. Mobile phase A and mobile phase B consisted of H2O and acetonitrile, both containing 0.1% of formic acid. A gradient elution was performed at a flow rate of 400 µl min-1 along 14 min as follows: initial conditions of 98% of mobile phase A was maintained for 1 min, and then decreased until 75% in 2 min, 50% in 3 min and 5% in 3 more min. 95% of mobile phase B was held for 3 min and then, a 0.55 min gradient was used to return to the initial conditions, which were held for 2.5 min to totally column recovery. |
Instrument Name: | Agilent 6550 |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Column Temperature: | 40 |
Flow Gradient: | initial conditions of 98% of mobile phase A was maintained for 1 min, and then decreased until 75% in 2 min, 50% in 3 min and 5% in 3 more min. 95% of mobile phase B was held for 3 min and then, a 0.55 min gradient was used to return to the initial conditions, which were held for 2.5 min to totally column recovery |
Flow Rate: | 400ul/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |