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MB Sample ID: SA152156
| Local Sample ID: | mv08618_47.mzXML |
| Subject ID: | SU001739 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Pooled |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002713 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 6550 |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | EI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6550 QTOF |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH002002 |
| Chromatography Summary: | The metabolomic analysis was carried out using an Ultra-Performance Liquid Chromatography (UPLC) system coupled to an iFunnel Q-ToF (quadrupole-time-of-flight) Agilent 6550 mass spectrometer (Agilent Technologies, CA, USA) using an UPLC BEH C18 (100 x 2.1 mm, 1.7 µm, Waters, Wexford, Ireland) column from Waters (Wexford, Ireland). Autosampler and column temperatures were set to 4 °C and 40 °C, respectively, and the injection volume was 5 µl. Mobile phase A and mobile phase B consisted of H2O and acetonitrile, both containing 0.1% of formic acid. A gradient elution was performed at a flow rate of 400 µl min-1 along 14 min as follows: initial conditions of 98% of mobile phase A was maintained for 1 min, and then decreased until 75% in 2 min, 50% in 3 min and 5% in 3 more min. 95% of mobile phase B was held for 3 min and then, a 0.55 min gradient was used to return to the initial conditions, which were held for 2.5 min to totally column recovery. |
| Instrument Name: | Agilent 6550 |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | initial conditions of 98% of mobile phase A was maintained for 1 min, and then decreased until 75% in 2 min, 50% in 3 min and 5% in 3 more min. 95% of mobile phase B was held for 3 min and then, a 0.55 min gradient was used to return to the initial conditions, which were held for 2.5 min to totally column recovery |
| Flow Rate: | 400ul/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
