Return to study ST001723 main page
MB Sample ID: SA162227
Local Sample ID: | Plasma_pos_83 |
Subject ID: | SU001800 |
Subject Type: | Mammal |
Subject Species: | Sus scrofa |
Taxonomy ID: | 9823 |
Age Or Age Range: | 0-125 days |
Weight Or Weight Range: | 1-80 kg |
Gender: | Male |
Animal Animal Supplier: | Swine Innovation Centre Sterksel, the Netherlands |
Animal Housing: | Metabolism cages |
Animal Light Cycle: | 12h light / 12h darknes |
Animal Feed: | Experimental diets (see publication) |
Animal Water: | Ad libitum |
Animal Inclusion Criteria: | Birth weight |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002807 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 RS |
Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Bruker Impact HD |
Ion Mode | POSITIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH002074 |
Chromatography Summary: | Chromatographic separation was performed on a Dionex UltiMate 3000RSL Binary UHPLC System (Thermo Scientific Dionex, Sunnyvale, CA) equipped with a HSS T3 C18 UHPLC column, 1.8 µm, 100x2.1 mm (Waters Corporation, Milford, MA). The column was maintained at 30°C. Samples were kept in the autosampler at 10°C, and the injection volume for both sample types was 3 µl. The mobile phases were 0.1% formic acid in Milli-Q water (A) and 0.1% formic acid in acetonitrile (B). The gradient program for the plasma samples was as follows: 0-12 min, linear gradient from 5-100% B; 12-13 min, 100% B and return to initial conditions in 0.2 min. In all cases, the column was re-equilibrated at 5% B for 2 min at the beginning of each run. The flow rate was 0.4 ml/min |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 30 |
Flow Rate: | 0.4 ml/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |