Return to study ST001733 main page
MB Sample ID: SA162649
| Local Sample ID: | POL_19 |
| Subject ID: | SU001810 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002821 | AN002822 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1200 | Agilent 1200 |
| Column | Discovery HS C18 (150 x 2.1mm,3.0um) | Discovery HS C18 (150 x 2.1mm,3.0um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6520 QTOF | Agilent 6520 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH002086 |
| Chromatography Summary: | Compound separation was performed on an Agilent HPLC system (1200 series, Agilent Technologies, Waldbronn, Germany) equipped with a degasser, two binary pumps, and a thermostated auto sampler. A volume of 10 μL of sample were injected into a Discovery HS C18 column (2.1 mm × 150 mm, 3.0 μm; Supelco, Sigma Aldrich, Germany), with a guard column Discovery® HS C18 (2 cm × 2.1 mm, 3 μm; Supelco, Sigma Aldrich, Germany), both maintained at 40 ℃. The flow rate was set at 0.6 mL/min. The elution gradient involved a mobile phase consisting of: (A) 0.1% formic acid (FA) in water, and (B) 0.1% FA in acetonitrile. Initial conditions were set at 25% phase B, which increased to 95% phase B in 35 min; then, it was re-equilibrated for 1 min and finally held for 9 min in the initial conditions. |
| Instrument Name: | Agilent 1200 |
| Column Name: | Discovery HS C18 (150 x 2.1mm,3.0um) |
| Column Temperature: | 40ºC |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
