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MB Sample ID: SA162667
Local Sample ID: | POL-06 |
Subject ID: | SU001811 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Combined analysis:
Analysis ID | AN002823 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1260 |
Column | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6470 QQQ |
Ion Mode | NEGATIVE |
Units | ug/mg |
Chromatography:
Chromatography ID: | CH002087 |
Chromatography Summary: | We used HPLC system (1260 Infinity, Agilent Technologies). Metabolites were separated on a Kinetex hydrophilic interaction liquid chromatography (HILIC) silica column (150 mm × 2.1 mm, particle size 100Å, Phenomenex, USA) maintained at 25 ºC. The mobile phases consisted of A) water, and B) acetonitrile, both with 7.5 mM ammonium acetate and 0.1% acetic acid, with a final pH of 4.0 in the aqueous phase. The flow rate was 0.5 mL/min with an injection volume of 5 µl. Gradient started with 5% of A for 2 min, then increased up to 50% until 12 min, and back to initial conditions until 22 min. |
Instrument Name: | Agilent 1260 |
Column Name: | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
Column Temperature: | 25ºC |
Flow Rate: | 0.5 mL/min |
Solvent A: | 100% water; 0.1% acetic acid; 7.5 mM ammonium acetate, pH 4 |
Solvent B: | 100% acetonitrile; 0.1% acetic acid; 7.5 mM ammonium acetate |
Chromatography Type: | HILIC |