Return to study ST001734 main page
MB Sample ID: SA162669
| Local Sample ID: | POL-04 |
| Subject ID: | SU001811 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002823 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 |
| Column | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6470 QQQ |
| Ion Mode | NEGATIVE |
| Units | ug/mg |
Chromatography:
| Chromatography ID: | CH002087 |
| Chromatography Summary: | We used HPLC system (1260 Infinity, Agilent Technologies). Metabolites were separated on a Kinetex hydrophilic interaction liquid chromatography (HILIC) silica column (150 mm × 2.1 mm, particle size 100Å, Phenomenex, USA) maintained at 25 ºC. The mobile phases consisted of A) water, and B) acetonitrile, both with 7.5 mM ammonium acetate and 0.1% acetic acid, with a final pH of 4.0 in the aqueous phase. The flow rate was 0.5 mL/min with an injection volume of 5 µl. Gradient started with 5% of A for 2 min, then increased up to 50% until 12 min, and back to initial conditions until 22 min. |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
| Column Temperature: | 25ºC |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 100% water; 0.1% acetic acid; 7.5 mM ammonium acetate, pH 4 |
| Solvent B: | 100% acetonitrile; 0.1% acetic acid; 7.5 mM ammonium acetate |
| Chromatography Type: | HILIC |
