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MB Sample ID: SA163539
Local Sample ID: | scfa_Exp2_13_Hu+GUT108_week4 |
Subject ID: | SU001825 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | GF 129SvEv background IL-10-deficient mice (Il10-/-); Il10-eGFP-reporter (Il10+/eGFP) mice (on a C57BL/6J background) |
Age Or Age Range: | 8-12 week old |
Animal Animal Supplier: | 8-12 week-age GF 129SvEv background IL-10-deficient mice (Il10-/-) were obtained from University of North Carolina National Gnotobiotic Rodent Resource Center; Il10-eGFP-reporter (Il10+/eGFP) mice on a C57BL/6J background mice were originally provided by Dr. C. L. Karp (Global Health, Bill & Melinda Gates Foundation, USA) |
Animal Housing: | Germ-free and gnotobiotic conditions at National Gnotobiotic Rodent Resource Center, University of North Carolina |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002845 | AN002846 | AN002847 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | GC | Reversed phase | Reversed phase |
Chromatography system | Agilent 7820A | Thermo Vanquish | Thermo Vanquish |
Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH C18 (150 x 2mm,1.7um) |
MS Type | EI | ESI | ESI |
MS instrument type | Single quadrupole | Orbitrap | Triple quadrupole |
MS instrument name | Agilent 5977B | Thermo Q Exactive Orbitrap | Thermo Quantis QQQ |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
Units | μmol/kg | ng/g | mg/g |
Chromatography:
Chromatography ID: | CH002106 |
Chromatography Summary: | Generic DB-5ms GC capillary column for analysis of SCFA derivatives. |
Instrument Name: | Agilent 7820A |
Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
Chromatography Type: | GC |
Chromatography ID: | CH002107 |
Chromatography Summary: | C18 LC (HSS-T3) for quantitation of tryptophan metabolites |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH002108 |
Chromatography Summary: | C18 LC (BEH C18) and (HSS T3) for quantitation of bile acids |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters Acquity BEH C18 (150 x 2mm,1.7um) |
Chromatography Type: | Reversed phase |