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MB Sample ID: SA164944
Local Sample ID: | S4R3 |
Subject ID: | SU001853 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002883 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 RS |
Column | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | arbitray unit |
Chromatography:
Chromatography ID: | CH002138 |
Chromatography Summary: | The UHPLC system was equipped with a binary pump, an autosampler and a column thermostat. The autosampler was set at 8 °C. The column oven temperature was 30 °C. Mobile phase A was 98% acetonitrile, 2% water and 0.1% formic acid while mobile phase B was 98% water, 2% acetonitrile, 30 mM ammonium formate and 0.1% formic acid. The mobile phase was freshly prepared before use. The optimized stepwise linear gradient (10% B in the first 2 min, 10% - 30% B in the next 5 min, followed by 30% - 60% in 3 min, and finally 60% - 90% in 5 min. |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
Column Temperature: | 25 |
Solvent A: | 98% acetonitrile/2% water; 0.1% formic acid |
Solvent B: | 98% water/2% acetonitrile; 0.1% formic acid; 30 mM ammonium formate |
Sample Loop Size: | 10 |
Chromatography Type: | HILIC |