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MB Sample ID: SA164964
| Local Sample ID: | S12R2 |
| Subject ID: | SU001853 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002883 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 RS |
| Column | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | arbitray unit |
Chromatography:
| Chromatography ID: | CH002138 |
| Chromatography Summary: | The UHPLC system was equipped with a binary pump, an autosampler and a column thermostat. The autosampler was set at 8 °C. The column oven temperature was 30 °C. Mobile phase A was 98% acetonitrile, 2% water and 0.1% formic acid while mobile phase B was 98% water, 2% acetonitrile, 30 mM ammonium formate and 0.1% formic acid. The mobile phase was freshly prepared before use. The optimized stepwise linear gradient (10% B in the first 2 min, 10% - 30% B in the next 5 min, followed by 30% - 60% in 3 min, and finally 60% - 90% in 5 min. |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
| Column Temperature: | 25 |
| Solvent A: | 98% acetonitrile/2% water; 0.1% formic acid |
| Solvent B: | 98% water/2% acetonitrile; 0.1% formic acid; 30 mM ammonium formate |
| Sample Loop Size: | 10 |
| Chromatography Type: | HILIC |
