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MB Sample ID: SA169200
Local Sample ID: | 56 |
Subject ID: | SU001900 |
Subject Type: | Mammal |
Subject Species: | Equus caballus |
Taxonomy ID: | 9796 |
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Combined analysis:
Analysis ID | AN002959 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Xcalibur |
Column | Phenomenex Synergi Fusion (150mm x 2mm,4um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap |
Ion Mode | POSITIVE |
Units | peak intensity |
Chromatography:
Chromatography ID: | CH002192 |
Chromatography Summary: | Samples were maintained at 4 °C before injection. The injection volume was 10 µL. Chromatographic separation was achieved on a Synergi Fusion 4µm, 150 mm x 2 mm reverse phase column (Phenomenex, Torrance, CA) maintained at 30 °C using a solvent gradient method. Solvent A was water (0.1% formic acid). Solvent B was methanol (0.1% formic acid). The gradient method used was 0-5 min (10% B to 40% B), 5-7 min (40% B to 95% B), 7-9 min (95% B), 9-9.1 min (95% B to 10% B), 9.1-13 min (10% B). The flow rate was 0.4 mL min-1. |
Instrument Name: | Thermo Xcalibur |
Column Name: | Phenomenex Synergi Fusion (150mm x 2mm,4um) |
Column Temperature: | 30 |
Flow Gradient: | 0-5 min (10% B to 40% B), 5-7 min (40% B to 95% B), 7-9 min (95% B), 9-9.1 min (95% B to 10% B), 9.1-13 min (10% B). |
Flow Rate: | 0.4 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Chromatography Type: | Reversed phase |