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MB Sample ID: SA169297
| Local Sample ID: | ODBS1_1 |
| Subject ID: | SU001901 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 53 ± 15 |
| Weight Or Weight Range: | 116 ± 25 |
| Gender: | Male and female |
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Combined analysis:
| Analysis ID | AN002960 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent Infinity 1290 |
| Column | Waters ACQUITY UPLC BEH C18 |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6545 QTOF |
| Ion Mode | POSITIVE |
| Units | Area |
Chromatography:
| Chromatography ID: | CH002193 |
| Chromatography Summary: | An ultra-high-performance liquid chromatography electrospray ionization quadrupole time-of-flight (UHPLC-ESI-Q-TOF-MS) were used to analyze the samples on positive ionization mode. Samples were analyzed by duplicate. UHPLC system was an Agilent Infinity 1290 provided by Agilent Technologies (Santa Clara, CA, USA) equipped with a multisampler (kept at -10 °C). Needle wash solutions were performed with 10% DCM in MeOH and ACN:MeOH:IPA:H2O (1:1:1:1, v/v/v/v), and 0.1% of HCOOH after each injection for 7.5 s. System were equipped with a column thermostat (maintained at 50 °C) and a quaternary solvent manager. An ACQUITY UPLC BEH C18 column was used for separations (2.1 mm x 100 mm, particle size 1.7 μm) purchased at Waters (Milford, CT, USA). Injection volume was 1 μl and flow rate was established at 0.4 ml/min. Mobile phases were composed of (A) H2O + NH4AC 10 mM + 0.1 % HCOOH and (B) ACN: IPA (1:1, v/v) + NH4AC 10 mM + 0.1 % HCOOH. Gradient was from 0 to 2 min 35-80 % B, 2 min to 7 min 80-100 % B and 7 to 14 min 100 % B. A re-equilibration of 7 min was performed after each run to bring system to initial conditions (35 % B). |
| Instrument Name: | Agilent Infinity 1290 |
| Column Name: | Waters ACQUITY UPLC BEH C18 |
| Column Temperature: | 50 |
| Flow Gradient: | 0 to 2 min 35-80 % B, 2 min to 7 min 80-100 % B and 7 to 14 min 100 % B |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 100% water; 0.1% formic acid; 10 mM ammonium acetate |
| Solvent B: | 50% acetonitrile/50% isopropanol; 0.1% formic acid; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
