Return to study ST001837 main page
MB Sample ID: SA170900
Local Sample ID: | 1024038821 |
Subject ID: | SU001914 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002979 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | UPLC (Waters Acquity, Waters Corporation, Milford, USA) |
Column | Agilent Zorbax Eclipse XDB C18 (100 x 3.0mm,3.5um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap |
Ion Mode | NEGATIVE |
Units | µM |
Chromatography:
Chromatography ID: | CH002208 |
Chromatography Summary: | LC - Instrument Parameters AbsoluteIDQ® p180 Kit (Biocrates Life Science AG, Innsbruck, Austria) System: UPLC (Waters Acquity, Waters Corporation, Milford, USA) Column: Agilent, Zorbax Eclipse XDB C18, 3.0 x 100 mm, 3.5 μM, Agilent Waldbronn, Germany Precolumn: Security Guard, Phenomenex, C18, 4 x 3 mm; Phenomenex, Aschaffenburg, Germany Materials: Water MiliQ, Methanol (Merck KGaA, Darmstadt, Germany, hypergrade for LC-MS) Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS) Ammonium Acetate (Honeywell - Fluka, Seelze, Germany) Formic Acid (Honeywell, Fluka, Seelze, Germany) Running Solvent: 5mM ammonium acetat in methanol LC: A: 2mL Formic acid in MilliQ water B: 1mL Formic Acid in Acetonitrile Gradient Table LC Time (min) Flow Rate (mL/min) %A %B Curve Initial 0.500 100.0 0.0 Initial 0.50 0.500 100.0 0.0 6 4.00 0.500 30.0 70.0 6 5.30 0.500 30.0 70.0 6 5.40 0.500 100.0 0.0 6 7.30 0.500 100.0 0.0 6 |
Methods Filename: | LC_parameters.pdf |
Instrument Name: | UPLC (Waters Acquity, Waters Corporation, Milford, USA) |
Column Name: | Agilent Zorbax Eclipse XDB C18 (100 x 3.0mm,3.5um) |
Flow Gradient: | FIA Time (min) Flow Rate (mL/min) %A %B Curve Initial 0.030 0.0 100.0 Initial 1.60 0.030 0.0 100.0 6 2.40 0.200 0.0 100.0 6 2.80 0.200 0.0 100.0 6 3.00 0.030 0.0 100.0 6 |
Flow Rate: | 0.03ml/min |
Solvent A: | 100% methanol; 5mM ammonium acetate |
Solvent B: | 50% methanol/50% water; 5mM ammonium acetate |
Chromatography Type: | Reversed phase |