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MB Sample ID: SA171053
| Local Sample ID: | BRN_ctrl_g2_Intra13_6h |
| Subject ID: | SU001915 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | albino C57BL/6 |
| Age Or Age Range: | 6-8 weeks |
| Gender: | Female |
| Animal Animal Supplier: | Jackson Laboratories (Bar Harbor, ME) |
| Animal Housing: | NCI-Bethesda Animal Facility |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002980 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 Infinity II |
| Column | Agilent AdvanceBio Glycan Map 2.1 x 100 mm 2.7µm column |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6545 |
| Ion Mode | POSITIVE |
| Units | ng |
Chromatography:
| Chromatography ID: | CH002209 |
| Chromatography Summary: | Samples were injected at 8 µL over an 8.3 min gradient on the AdvanceBio Glycan Map 2.1 x 100 mm 2.7µm column at 35°C with a flow rate of 0.220 mL/min. The LC gradient only utilized LC/MS grade reagents when preparing mobile phases, A (88:12 H2O/acetonitrile (ACN) and B 90% ACN (aq). Both mobile phases were composed with 10 mM ammonium acetate and titrated to pH 6.85 using formic acid and ammonium hydroxide. The LC gradient was initially 100% B for 0.25 min and then ramped to 55% B at 2.5 min; 49% B at 4.5 min; 35% B at 5.5 min; 20% B at 6 min; held for 0.5 min; 15% B at 7 min; 100%B at 8.3 min followed by equilibration for 1.2 min. |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent AdvanceBio Glycan Map 2.1 x 100 mm 2.7µm column |
| Column Pressure: | 600 bar |
| Column Temperature: | 35°C |
| Flow Gradient: | The LC gradient was initially 100% B for 0.25 min and then ramped to 55% B at 2.5 min; 49% B at 4.5 min; 35% B at 5.5 min; 20% B at 6 min; held for 0.5 min; 15% B at 7 min; 100%B at 8.3 min followed by equilibration for 1.2 min. |
| Flow Rate: | 0.220 mL/min |
| Injection Temperature: | 4°C |
| Internal Standard: | Continuous accurate mass correction was achieved by infusing proprietary Agilent Technologies API-TOF reference mass standard solution |
| Retention Time: | 2.5-2.7 min |
| Sample Injection: | 8 uL |
| Sampling Cone: | 2kV |
| Solvent A: | 88% water/12% acetonitrile; 10 mM ammonium acetate, pH 6.85 |
| Solvent B: | 90% acetonitrile/10% water; 10 mM ammonium acetate, pH 6.85 |
| Analytical Time: | 8.3 min |
| Capillary Voltage: | 3 kV |
| Sheath Liquid: | N2 |
| Chromatography Type: | HILIC |
