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MB Sample ID: SA173172
| Local Sample ID: | Alistipes onderdonkii St.17-1 (50uM_3-oxo-Δ4-LCA_pH9) |
| Subject ID: | SU001929 |
| Subject Type: | Bacteria |
| Subject Species: | Bacteria |
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Combined analysis:
| Analysis ID | AN003000 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu CBM20A |
| Column | InertSustain C18 |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Shimadzu LCMS-8040 |
| Ion Mode | UNSPECIFIED |
| Units | uM |
Chromatography:
| Chromatography ID: | CH002225 |
| Chromatography Summary: | 2 μL of the solution was injected to LC/ESI-MS/MS (LC-MS8040 tandem mass spectrometer, equipped with an ESI probe and Nexera X2 ultra-high-pressure liquid chromatography system; Shimadzu). A separation column, InertSustain C18 (150 mm × 2.1 mm ID, 2 μm particle size; GL Sciences Inc.), was utilized at 40 °C. Mixture A (10 mM ammonium acetate, 0.01% formic acid, and 20% acetonitrile) and mixture B (30% acetonitrile and 70% methanol) were used as the eluent, and the separation was carried out by linear gradient elution at a flow rate of 0.2 mL/min. The mobile phase composition was gradually changed as follows: Mixture A:B (80:20, v/v) for 0.1 min, (48:52, v/v) for 0.1-1 min, (30:70, v/v) for 1-27 min, (0:100, v/v) for 27-27.1 min, (0:100, v/v) for 27.1-33 min, (80:20, v/v) for 33-33.1 min, and (80:20 v/v) for 33.1-83 min. The total run time was 38 min. |
| Instrument Name: | Shimadzu CBM20A |
| Column Name: | InertSustain C18 |
| Column Temperature: | 40 |
| Flow Gradient: | The mobile phase composition was gradually changed as follows: Mixture A:B (80:20, v/v) for 0.1 min, (48:52, v/v) for 0.1-1 min, (30:70, v/v) for 1-27 min, (0:100, v/v) for 27-27.1 min, (0:100, v/v) for 27.1-33 min, (80:20, v/v) for 33-33.1 min, and (80:20 v/v) for 33.1-83 min. The total run time was 38 min. |
| Flow Rate: | 0.2ml/min |
| Solvent A: | 20% acetonitrile/80% water; 0.01% formic acid; 10mM ammonium acetate |
| Solvent B: | 30% acetonitrile/70% methanol |
| Chromatography Type: | Reversed phase |
