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MB Sample ID: SA173649
Local Sample ID: | Lachnospiraceae St.58-2 (50uM_3-oxo-Δ4-LCA_pH7) |
Subject ID: | SU001929 |
Subject Type: | Bacteria |
Subject Species: | Bacteria |
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Combined analysis:
Analysis ID | AN003000 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu CBM20A |
Column | InertSustain C18 |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Shimadzu LCMS-8040 |
Ion Mode | UNSPECIFIED |
Units | uM |
Chromatography:
Chromatography ID: | CH002225 |
Chromatography Summary: | 2 μL of the solution was injected to LC/ESI-MS/MS (LC-MS8040 tandem mass spectrometer, equipped with an ESI probe and Nexera X2 ultra-high-pressure liquid chromatography system; Shimadzu). A separation column, InertSustain C18 (150 mm × 2.1 mm ID, 2 μm particle size; GL Sciences Inc.), was utilized at 40 °C. Mixture A (10 mM ammonium acetate, 0.01% formic acid, and 20% acetonitrile) and mixture B (30% acetonitrile and 70% methanol) were used as the eluent, and the separation was carried out by linear gradient elution at a flow rate of 0.2 mL/min. The mobile phase composition was gradually changed as follows: Mixture A:B (80:20, v/v) for 0.1 min, (48:52, v/v) for 0.1-1 min, (30:70, v/v) for 1-27 min, (0:100, v/v) for 27-27.1 min, (0:100, v/v) for 27.1-33 min, (80:20, v/v) for 33-33.1 min, and (80:20 v/v) for 33.1-83 min. The total run time was 38 min. |
Instrument Name: | Shimadzu CBM20A |
Column Name: | InertSustain C18 |
Column Temperature: | 40 |
Flow Gradient: | The mobile phase composition was gradually changed as follows: Mixture A:B (80:20, v/v) for 0.1 min, (48:52, v/v) for 0.1-1 min, (30:70, v/v) for 1-27 min, (0:100, v/v) for 27-27.1 min, (0:100, v/v) for 27.1-33 min, (80:20, v/v) for 33-33.1 min, and (80:20 v/v) for 33.1-83 min. The total run time was 38 min. |
Flow Rate: | 0.2ml/min |
Solvent A: | 20% acetonitrile/80% water; 0.01% formic acid; 10mM ammonium acetate |
Solvent B: | 30% acetonitrile/70% methanol |
Chromatography Type: | Reversed phase |