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MB Sample ID: SA174893
Local Sample ID: | KO_3 |
Subject ID: | SU001954 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Combined analysis:
Analysis ID | AN003080 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Accela 1250 |
Column | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo Quantum Ultra |
Ion Mode | POSITIVE |
Units |
Chromatography:
Chromatography ID: | CH002252 |
Chromatography Summary: | Samples were analyzed with a Quantum Ultra triple quadrupole mass spectrometer connected to an Accela HPLC and Accela autosampler using a solvent gradient. Ceramides identity was achieved through MRM analysis with soft fragmentation. Quantitative analysis is based on calibration curves generated for each ceramide. J. Bielawski et al. / Methods 39 (2006) 82–91 |
Instrument Name: | Thermo Accela 1250 |
Column Name: | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
Chromatography Type: | Reversed phase |