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MB Sample ID: SA176217
| Local Sample ID: | MP07 |
| Subject ID: | SU001978 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J, C57BL/6J.rag1−/− |
| Age Or Age Range: | 6-8 weeks |
| Gender: | Female |
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Combined analysis:
| Analysis ID | AN003087 | AN003088 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | Agilent Zorbax HILIC Plus RRHD (100 x 2.1mm,1.8um,95Å) | Agilent Zorbax HILIC Plus RRHD (100 x 2.1mm,1.8um,95Å) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH002279 |
| Chromatography Summary: | Metabolite separation was performed on a Zorbax HILIC Plus RRHD (95Å, 1.8 µm, 2.1x100mm) analytical column connected to a 3 x 5 mm Zorbax HILIC Plus UHPLC guard column. The autosampler and column temperature were set to 4°C and 40°C, respectively. In positive and negative mode, eluent A was 10 mM ammonium acetate (pH neutral) in acetonitrile/milliQ water (95:5, v/v) and eluent B was 10 mM ammonium acetate (pH neutral) in acetonitrile/milliQ water (50:50, v/v). Total method runtime was 12 min with the following gradient for both modes: 0 min (1% eluent B) - 3.5 min (50% B) - 5.5 min (99%B) - 6.5 min (99% B) - 6.7 min (1% B) - 12 min (1% B). Flow rate was set to 0.5 mL/min. |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent Zorbax HILIC Plus RRHD (100 x 2.1mm,1.8um,95Å) |
| Column Temperature: | 40 |
| Flow Gradient: | Total method runtime was 12 min with the following gradient for both modes: 0 min (1% eluent B) - 3.5 min (50% B) - 5.5 min (99%B) - 6.5 min (99% B) - 6.7 min (1% B) - 12 min (1% B). Flow rate was set to 0.5 mL/min. |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 95% acetonitrile/5% water; 10 mM ammonium acetate,pH neutral |
| Solvent B: | 50% acetonitrile/50% water; 10 mM ammonium acetate,pH neutral |
| Chromatography Type: | HILIC |
