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MB Sample ID: SA176600
| Local Sample ID: | OMV-1524-1 |
| Subject ID: | SU001982 |
| Subject Type: | Bacteria |
| Subject Species: | Bacteroides thetaiotaomicron |
| Taxonomy ID: | 818 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003101 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 6550 |
| Column | Thermo Betasil C18 (100 x 2.1mm,5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6550 QTOF |
| Ion Mode | UNSPECIFIED |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH002288 |
| Chromatography Summary: | LC/MS analyses were conducted on an Agilent 6550 A QTOF instrument with an Agilent 1290 HPLC with autosampler, operated by Agilent Masshunter software (Santa Clara, CA USA). Separation of the total lipid extracts was achieved by a Thermo (Waltham MA, USA) 100 × 2.1 mm BETASIL 5 μm C18 column at a flow rate of 300 μl/min at room temperature. The mobile phase contained 5 mM ammonium formate (pH 5.0) both in solvent A, acetonitrile:water (60:40, v/v), and solvent B, isopropanol:acetonitrile (90:10, v/v). A gradient elution in the following manner was applied: 68% A, 0–1.5 min; 68–55% A, 1.5–4 min; 55–48% A, 4–5 min; 48–42% A, 5–8 min; 42–34% A, 8–11 min; 34–30% A, 11–14 min; 30–25% A, 14–18 min; 25–3% A, 18–23 min; 3–0% A, 25–30 min; 0% A, 30–35 min; 68% A, 35–40 min. Both the positive-ion and negative-ion ESI MS scans were acquired in the mass range of 200-2000 Da at a rate of 2 scans/min. |
| Instrument Name: | Agilent 6550 |
| Column Name: | Thermo Betasil C18 (100 x 2.1mm,5um) |
| Column Temperature: | RT |
| Flow Gradient: | 68% A, 0-1.5 min; 68-55% A, 1.5-4 min; 55-48% A, 4-5 min; 48-42% A, 5-8 min; 42-34% A, 8-11 min; 34-30% A, 11-14 min; 30-25% A, 14-18 min; 25-3% A, 18-23 min; 3-0% A, 25-30 min; 0% A, 30-35 min; 68% A, 35-40 min |
| Flow Rate: | 300ul/min |
| Solvent A: | 60% acetonitrile/40% water; 5 mM ammonium formate, pH 5.0 |
| Solvent B: | 90% isopropanol/10% acetonitrile |
| Chromatography Type: | Reversed phase |
