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MB Sample ID: SA184585
| Local Sample ID: | post-differentiation_later-1 |
| Subject ID: | SU002038 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 51.3±5 |
| Gender: | Female |
| Cell Biosource Or Supplier: | Promocell (GmbH, Heidelberg, Germany) and Zen-Bio (Zen-Bio, Inc., Research Triangle Park, NC, USA) |
| Cell Strain Details: | Human subcutaneous white adipocytes |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003193 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Thermo Acclaim 120 C18 (150 x 2.1mm,3um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | normalized area values |
Chromatography:
| Chromatography ID: | CH002361 |
| Chromatography Summary: | We used a set of linear gradients starting at 20% of solvent B and increasing linearly to 100% over 50 min, maintained at 100% solvent B for 60 min, then decreased linearly to 20% B from 60 min to 60.1 min, and finished with 20% solvent B for the last 10 min. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Acclaim 120 C18 (150 x 2.1mm,3um) |
| Column Temperature: | 50 °C |
| Flow Rate: | 300 μL/min |
| Solvent A: | 50% water/25% methanol/25% acetonitrile; 0.1% formic acid; 5 mM ammonium formate |
| Solvent B: | 90% acetonitrile/ 10% water; 0.1% formic acid; 5 mM ammonium formate |
| Chromatography Type: | Reversed phase |
