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MB Sample ID: SA192264
| Local Sample ID: | NHF-96 |
| Subject ID: | SU002126 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003325 | AN003326 | AN003327 | AN003328 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters Xbridge BEH HILIC (75 x 2.1mm,2.5um) | Waters Xbridge BEH HILIC (75 x 2.1mm,2.5um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Fusion Orbitrap | Thermo Fusion Orbitrap | Thermo Fusion Orbitrap | Thermo Fusion Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | peak height | peak height | peak height | peak height |
Chromatography:
| Chromatography ID: | CH002464 |
| Chromatography Summary: | For hydrophilic interaction (HILIC) chromatography, 5 µl of the prepared samples were injected for analysis. An ultimate 3000 UPLC XBridge BHE Amide column (2.1 x 100 mm, 2.5 µm; Waters Corporation) was used and maintained at 35℃ for separation. The mobile phase was delivered at 0.35mL/min. The mobile phase A consisted of 20mM ammonium acetate in the water of pH 9.0 and mobile phase B was 100% acetonitrile. The elution gradient is used as follows: 0 min-85% B, 2 min-10% B, 14 min- 10% B, 14.1 min- 85% B, 16 min- 85%B. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Xbridge BEH HILIC (75 x 2.1mm,2.5um) |
| Column Temperature: | 35 |
| Flow Gradient: | 0 min-85% B, 2 min-10% B, 14 min- 10% B, 14.1 min- 85% B, 16 min- 85%B. |
| Flow Rate: | 0.35mL/min |
| Solvent A: | 100% water; 20 mM ammonium acetate, pH 9.0 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH002465 |
| Chromatography Summary: | For reversed-phase (RP) chromatographic analyses, 5 µL of prepared samples were injected. The separation was performed using a UPLC ultimate 3000 using the HSS T3 column (2.1x 100mm, 1.7 µm; Waters Corporation) at 40℃. The mobile phase was delivered at 300µL/min and consisted of eluent A (water with 0.1%formic acid) and eluent B (acetonitrile with 0.1% formic acid), delivered in a gradient profile: 0min-1% B, 1min-15%B, 4min-35% B, 7min-95% B, 9 min- 95% B, 10min-1% B, 14 min- 1% B. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Flow Gradient: | 0min-1% B, 1min-15%B, 4min-35% B, 7min-95% B, 9 min- 95% B, 10min-1% B, 14 min- 1% B |
| Flow Rate: | 300µL/min |
| Solvent A: | 100% water; 0.1%formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
