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MB Sample ID: SA192411
| Local Sample ID: | exp6_hilic_neg_02 |
| Subject ID: | SU002128 |
| Subject Type: | Invertebrate |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003330 | AN003331 | AN003332 | AN003333 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Scientific Accucore C30 (150 x 2.1mm,2.6um) | Thermo Scientific Accucore C30 (150 x 2.1mm,2.6um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH002467 |
| Chromatography Summary: | The following gradient program was used: -5.0 20% B; 0.0 min 20% B; 1.0 min 60% B; 5.0 min 70% B; 5.5 min 85% B; 8.0 min 90% B; 8.2-10.5 min 100% B; 10.7-12.0 min 20% B. A curve 5 value was set for -5.0 and 0.0 minutes, and a curve 6 for the remainder of the gradient. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Scientific Accucore C30 (150 x 2.1mm,2.6um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400 mL/min |
| Sample Injection: | 2.0 uL |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002468 |
| Chromatography Summary: | The following gradient program was used: -5.0 min 95% B; 0.0-0.5 min 95% B; 8.0-9.4 min 40% B; 9.5-11.0 min 95% B. A curve 5 value was set for -5.0 and 0.0 minutes, a curve 6 at 0.5 min, curve 7 at 8.0 min, and a curve 6 for the remainder of the gradient. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Rate: | 0.400 mL/min |
| Sample Injection: | 2.0 uL |
| Solvent A: | 80% water/20% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
