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MB Sample ID: SA193986
| Local Sample ID: | Hydrated_RPLC_Neg_1 |
| Subject ID: | SU002142 |
| Subject Type: | Plant |
| Subject Species: | Arabidopsis thaliana |
| Taxonomy ID: | 3702 |
| Genotype Strain: | Col-0 |
| Age Or Age Range: | Mature pollen, Pollen hydration Stage (45 min), Pollen germination stage (4 hours) |
| Species Group: | Pollen |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003354 | AN003355 | AN003356 | AN003357 | AN003358 | AN003359 |
|---|---|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Eksigent Ekspert microLC 200 | Eksigent Ekspert microLC 200 | Eksigent Ekspert microLC 200 | Eksigent Ekspert microLC 200 | Eksigent Ekspert microLC 200 | Eksigent Ekspert microLC 200 |
| Column | SeQuant Zic-HILIC (150 x 0.5mm,5um) | SeQuant Zic-HILIC (150 x 0.5mm,5um) | Higgins Analytica Targa C18 (150 x 0.3mm,3.5um) | Higgins Analytica Targa C18 (150 x 0.3mm,3.5um) | Machery-Nagel Nucleodur C8 (150 x 0.5mm,5um) | Machery-Nagel Nucleodur C8 (150 x 0.5mm,5um) |
| MS Type | ESI | ESI | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Intensity | Intensity | Intensity | Intensity | Intensity | Intensity |
Chromatography:
| Chromatography ID: | CH002483 |
| Methods Filename: | Chromatography_PollentMetabolomics.docx |
| Instrument Name: | Eksigent Ekspert microLC 200 |
| Column Name: | SeQuant Zic-HILIC (150 x 0.5mm,5um) |
| Column Temperature: | 35 |
| Flow Rate: | 0.015 |
| Solvent A: | 100% water; 10 mM ammonium bicarbonate |
| Solvent B: | 95% acetonitrile/5% water; 10 mM ammonium bicarbonate |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH002484 |
| Methods Filename: | Chromatography_PollentMetabolomics.docx |
| Instrument Name: | Eksigent Ekspert microLC 200 |
| Column Name: | Higgins Analytica Targa C18 (150 x 0.3mm,3.5um) |
| Column Temperature: | 35 |
| Flow Rate: | 0.015 |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002485 |
| Methods Filename: | Chromatography_PollenMetabolomics |
| Instrument Name: | Eksigent Ekspert microLC 200 |
| Column Name: | Machery-Nagel Nucleodur C8 (150 x 0.5mm,5um) |
| Column Temperature: | 35 |
| Flow Rate: | 0.04 |
| Solvent A: | 100% water; 0.1% acetic acid; 10 mM ammonium acetate |
| Solvent B: | 70% acetonitrile/30% isopropanol; 0.1% acetic acid; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
