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MB Sample ID: SA198284
Local Sample ID: | MC-04_2017-07-25 |
Subject ID: | SU002166 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 47-89 years |
Gender: | Male and female |
Human Inclusion Criteria: | Patients with lung cancer |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003396 | AN003397 | AN003398 | AN003399 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
Chromatography system | Agilent 6550 | Agilent 6550 | Agilent 6550 | Agilent 6550 |
Column | Waters Atlantis dC18 (100 x 3mm,3um) | Waters Atlantis dC18 (100 x 3mm,3um) | Waters BEH Amide (150 x 3.0mm,1.7um) | Waters BEH Amide (150 x 3.0mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | QTOF | QTOF | QTOF | QTOF |
MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF |
Ion Mode | NEGATIVE | POSITIVE | NEGATIVE | POSITIVE |
Units | Values are raw peak area | raw peak area | raw peak area | Values are raw peak area |
Chromatography:
Chromatography ID: | CH002511 |
Chromatography Summary: | LC method 1: employed an Atlantis dC18 column (3x100 mm, 3 µm, Waters, UK) maintained at 60 °C with flow rate at 0.4 mL/min. Mobile phases were (A) water and (B) methanol both containing 5 mmol/L ammonium formate and 0.1 % formic acid. The elution gradient started at 5 % B at 0 to 1 min increasing linearly to 100 % by 12 min, held at 100 % B until 14 min, returning to 95 % A for 5 min. |
Instrument Name: | Agilent 6550 |
Column Name: | Waters Atlantis dC18 (100 x 3mm,3um) |
Column Temperature: | 60 |
Flow Gradient: | The elution gradient started at 5 % B at 0 to 1 min increasing linearly to 100 % by 12 min, held at 100 % B until 14 min, returning to 95 % A for 5 min |
Flow Rate: | 0.4 mL/min |
Solvent A: | 100% water; 0.1 % formic acid; 5 mM ammonium formate |
Solvent B: | 100% methanol; 0.1 % formic acid; 5 mM ammonium formate |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH002512 |
Chromatography Summary: | LC method 2: used a BEH amide column (3x150 mm, 1.7 µm, Waters, UK) maintained at 40 °C with flow rate at 0.6 mL/min. Mobile phases were (A) water and (B) acetonitrile both containing 0.1 % formic acid. The elution gradient started at 99 % B, decreasing linearly to 30 % from 1 to 12 min, held at 30 % B until 12.6 min, returning to 99 % B for 3.4 min. Sample injection volume was 1 µL for both LC methods. |
Instrument Name: | Agilent 6550 |
Column Name: | Waters BEH Amide (150 x 3.0mm,1.7um) |
Column Temperature: | 40 |
Flow Gradient: | The elution gradient started at 99% B, decreasing linearly to 30% from 1 to 12 min, held at 30% B until 12.6 min, returning to 99% B for 3.4 min. |
Flow Rate: | 0.6 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | HILIC |