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MB Sample ID: SA205782
| Local Sample ID: | shctrl NG-3 |
| Subject ID: | SU002230 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003511 | AN003512 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Accucore C18 (100 x 2.1mm,2.6um) | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Fusion Orbitrap | Thermo Fusion Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | AUC/ngDNA | AUC/ngDNA |
Chromatography:
| Chromatography ID: | CH002592 |
| Chromatography Summary: | Mobile phase A (MPA) was 40:60 acetonitrile:0.1% formic acid in 10 mM ammonium formate. Mobile phase B (MPB) was 90:9:1 isopropanol: acetonitrile: 0.1% formic acid in 10 mM ammonium formate. The chromatographic method included a Thermo Fisher Scientific Accucore C30 column (2.6 μm, 150 x 2.1 mm) maintained at 40°C, a mobile phase flowrate of 0.200 mL/min, and a gradient elution program as follows: 0-3 min, 30% MPB; 3-13 min, 30-43% MPB; 13.1-33 min, 50-70% MPB; 33-48 min, 70-99% MPB; 48-55 min, 99% MPB; 55.1-60 min, 30% MPB. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0-3 min, 30% B; 3-13 min, 30-43% B; 13.1-33 min, 50-70% B; 33-48 min, 70-99% B; 48-55 min, 99% B; 55.1-60 min, 30% B |
| Flow Rate: | 0.200 mL/min |
| Solvent A: | 40% acetonitrile/60% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/9% acetonitrile/1% water; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
