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MB Sample ID: SA207013
Local Sample ID: | 161116_Smp_SA60_A |
Subject ID: | SU002245 |
Subject Type: | Cultured cells |
Subject Species: | Marine Plankton |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003535 | AN003536 | AN003537 | AN003538 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | HILIC | HILIC | HILIC | Reversed phase |
Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class |
Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | Waters Acquity UPLC HSS Cyano (100 x 2.1 mm,1.8um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Waters Xevo TQ-S | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | UNSPECIFIED | POSITIVE | NEGATIVE | POSITIVE |
Units | presence (1), absence (0), or background level (NA) | presence (1), absence (0), or background level (NA) | presence (1), absence (0), or background level (NA) | presence (1), absence (0), or background level (NA) |
Chromatography:
Chromatography ID: | CH002611 |
Chromatography Summary: | For HILIC chromatography, a SeQuant ZIC-pHILIC column (5 µm particle size, 2.1 mm x 150 mm, from Millipore) was used with 10 mM ammonium carbonate in 85:15 acetonitrile to water (Solvent A) and 10 mM ammonium carbonate in 60:40 water to acetonitrile (Solvent B) at a flow rate of 0.15 mL/min. |
Methods Filename: | CH_Ingalls_LC_protocol.pdf |
Instrument Name: | Waters Acquity I-Class |
Column Name: | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
Column Temperature: | 30 |
Flow Rate: | 0.15 mL/min |
Solvent A: | 85% acetonitrile/15% water; 10 mM ammonium carbonate |
Solvent B: | 40% acetonitrile/60% water; 10 mM ammonium carbonate |
Chromatography Type: | HILIC |
Chromatography ID: | CH002612 |
Chromatography Summary: | For reversed phase (RP) chromatography, a Waters Acquity UPLC HSS Cyano column (1.8 µm particle size, 2.1 mm x 100 mm) equipped with a Waters Acquity UPLC HSS Cyano guard column (1.8 µm particle size, 2.1 mm x 5 mm) was used with 0.1% formic acid in water (Solvent A) and 0.1% formic acid in acetonitrile (Solvent B) at a flow rate of 0.4 mL/ min. |
Methods Filename: | CH_Ingalls_LC_protocol.pdf |
Instrument Name: | Waters Acquity I-Class |
Column Name: | Waters Acquity UPLC HSS Cyano (100 x 2.1 mm,1.8um) |
Column Temperature: | 35 |
Flow Rate: | 0.4 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |