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MB Sample ID: SA207495
Local Sample ID: | 21 |
Subject ID: | SU002248 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Combined analysis:
Analysis ID | AN003542 | AN003543 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 6550 | Agilent 6550 |
Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6540 QTOF | Agilent 6545 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | pmol per sample | pmol per sample |
Chromatography:
Chromatography ID: | CH002616 |
Chromatography Summary: | Positive Mode RP LCMS |
Instrument Name: | Agilent 6550 |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Column Temperature: | 65 C |
Flow Gradient: | The chromatography gradient for both positive and negative modes started at 15% mobile phase B then increased to 30% B over 2.4 min. It sequentially increased to 48% B from 2.4 – 3.0 min, 82% B from 3 – 13.2 min, and 99% B from 13.2 – 13.8 min where it’s held until 16.7 min and returned to the initial conditions and equilibrated for 5 min. |
Flow Rate: | 0.4 mL min |
Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 90% isopropanol/9% acetonitrile/1% water;0.1% formic acid; 10 mM ammonium formate |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH002617 |
Chromatography Summary: | Negative Mode RP LCMS |
Instrument Name: | Agilent 6550 |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Column Temperature: | 65 C |
Flow Gradient: | The chromatography gradient for both positive and negative modes started at 15% mobile phase B then increased to 30% B over 2.4 min. It sequentially increased to 48% B from 2.4 – 3.0 min, 82% B from 3 – 13.2 min, and 99% B from 13.2 – 13.8 min where it’s held until 16.7 min and returned to the initial conditions and equilibrated for 5 min. |
Flow Rate: | 0.4 mL min |
Solvent A: | 40% water/60% acetonitrile; 10 mM ammonium formate |
Solvent B: | 90% isopropanol/9% acetonitrile/1% water; 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |