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MB Sample ID: SA210055
Local Sample ID: | HF_376_S15 |
Subject ID: | SU002272 |
Subject Type: | Other organism |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6N mice |
Age Or Age Range: | 5 weeks |
Gender: | Male |
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Combined analysis:
Analysis ID | AN003580 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Thermo Hypersil Gold (100 x 2. mm,1.9um) |
MS Type | ESI |
MS instrument type | QTRAP |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | NEGATIVE |
Units | time_m/z |
Chromatography:
Chromatography ID: | CH002647 |
Chromatography Summary: | Prepared samples were analyzed by RPLC-HRMS/MS in the Vanderbilt Center for Innovative Technology (CIT) using a modified version of a reversed phase chromatography negative ionization method. Metabolites were separated on a Thermo Fisher Scientific (Waltham, MA) Hypersil Gold C18 column (100 x 2.1 mm, 1.9 μm particle size) using water/acetonitrile gradient with formic acid (0.1%) added to both mobile phases. |
Instrument Name: | Thermo Vanquish |
Column Name: | Thermo Hypersil Gold (100 x 2. mm,1.9um) |
Column Temperature: | 40 |
Flow Rate: | 0.25 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 80% acetonitrile/20% water; 0.1% formic acid |
Chromatography Type: | Reversed phase |