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MB Sample ID: SA210057
| Local Sample ID: | HF_371_S11 |
| Subject ID: | SU002272 |
| Subject Type: | Other organism |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6N mice |
| Age Or Age Range: | 5 weeks |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003580 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Thermo Hypersil Gold (100 x 2. mm,1.9um) |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | NEGATIVE |
| Units | time_m/z |
Chromatography:
| Chromatography ID: | CH002647 |
| Chromatography Summary: | Prepared samples were analyzed by RPLC-HRMS/MS in the Vanderbilt Center for Innovative Technology (CIT) using a modified version of a reversed phase chromatography negative ionization method. Metabolites were separated on a Thermo Fisher Scientific (Waltham, MA) Hypersil Gold C18 column (100 x 2.1 mm, 1.9 μm particle size) using water/acetonitrile gradient with formic acid (0.1%) added to both mobile phases. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Hypersil Gold (100 x 2. mm,1.9um) |
| Column Temperature: | 40 |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 80% acetonitrile/20% water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
