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MB Sample ID: SA210655
Local Sample ID: | T3.40.LWS3_neg |
Subject ID: | SU002284 |
Subject Type: | Plant |
Subject Species: | Pinus pinaster |
Taxonomy ID: | 71647 |
Age Or Age Range: | one-two years |
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Combined analysis:
Analysis ID | AN003597 | AN003598 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Phenomenex Luna Omega Polar C18 (100 x 2.1 mm,1.7um) | Phenomenex Luna Omega Polar C18 (100 x 2.1 mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Bruker Impact II HD | Bruker Impact II HD |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH002658 |
Chromatography Summary: | A fifty-three-minute mobile phase gradient was employed. Gradient elution chromatography was performed starting with 100 % A to 98 % A in 1 min; hold for 9 min, gradient to 60 % A in 21 min, gradient to 45 % A in 5 min; hold for 2 min, gradient to 5 % A in 3 min; hold 5 min, return to initial conditions in 3 min and equilibrate for 5 min (total run time: 53 min). Solvent A was 100 % H2O containing 0.1 % formic acid, and solvent B was 100 % ACN containing 0.1 % formic acid. A flow rate of 0.1 mL/min was used. |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Phenomenex Luna Omega Polar C18 (100 x 2.1 mm,1.7um) |
Column Temperature: | 30 ÂșC |
Flow Gradient: | Gradient elution chromatography was performed starting with 100 % A to 98 % A in 1 min; hold for 9 min, gradient to 60 % A in 21 min, gradient to 45 % A in 5 min; hold for 2 min, gradient to 5 % A in 3 min; hold 5 min, return to initial conditions in 3 min and equilibrate for 5 min (total run time: 53 min) |
Flow Rate: | 0.1 mL/min |
Retention Time: | 53 min |
Sample Injection: | 5 uL |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Capillary Voltage: | 4.5 kV |
Washing Buffer: | IPA and Methanol |
Randomization Order: | True |
Chromatography Type: | Reversed phase |