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MB Sample ID: SA210661
| Local Sample ID: | T5.30.HWS1_neg |
| Subject ID: | SU002284 |
| Subject Type: | Plant |
| Subject Species: | Pinus pinaster |
| Taxonomy ID: | 71647 |
| Age Or Age Range: | one-two years |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003597 | AN003598 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Phenomenex Luna Omega Polar C18 (100 x 2.1 mm,1.7um) | Phenomenex Luna Omega Polar C18 (100 x 2.1 mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker Impact II HD | Bruker Impact II HD |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH002658 |
| Chromatography Summary: | A fifty-three-minute mobile phase gradient was employed. Gradient elution chromatography was performed starting with 100 % A to 98 % A in 1 min; hold for 9 min, gradient to 60 % A in 21 min, gradient to 45 % A in 5 min; hold for 2 min, gradient to 5 % A in 3 min; hold 5 min, return to initial conditions in 3 min and equilibrate for 5 min (total run time: 53 min). Solvent A was 100 % H2O containing 0.1 % formic acid, and solvent B was 100 % ACN containing 0.1 % formic acid. A flow rate of 0.1 mL/min was used. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Phenomenex Luna Omega Polar C18 (100 x 2.1 mm,1.7um) |
| Column Temperature: | 30 ºC |
| Flow Gradient: | Gradient elution chromatography was performed starting with 100 % A to 98 % A in 1 min; hold for 9 min, gradient to 60 % A in 21 min, gradient to 45 % A in 5 min; hold for 2 min, gradient to 5 % A in 3 min; hold 5 min, return to initial conditions in 3 min and equilibrate for 5 min (total run time: 53 min) |
| Flow Rate: | 0.1 mL/min |
| Retention Time: | 53 min |
| Sample Injection: | 5 uL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Capillary Voltage: | 4.5 kV |
| Washing Buffer: | IPA and Methanol |
| Randomization Order: | True |
| Chromatography Type: | Reversed phase |
