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MB Sample ID: SA214048

Local Sample ID:8_EA_2-pos
Subject ID:SU002325
Subject Type:Plant
Subject Species:Camelina Sativa
Age Or Age Range:10 days after fertilization
Species Group:Developing seeds

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Combined analysis:

Analysis ID AN003655 AN003656
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Dionex UltiMate 3000 RSLCnano Dionex UltiMate 3000 RSLCnano
Column Custom C8 - Higgins Analytical Custom C8 - Higgins Analytical
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Fusion Tribrid Orbitrap Thermo Fusion Tribrid Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Intensity Intensity

Chromatography:

Chromatography ID:CH002709
Chromatography Summary:Separations for lipidomics were achieved using the loading pump of a Dionex UltiMate 3000 RSLCnano system (Thermo Fisher Scientific) operating at a flow rate of 40 µL min-1 equipped with a custom-made C8 column (100 x 0.5 x 5 µm) from Higgins Analytical Inc. (Mountain view, CA) re-packed from a nucleodur C8 Gravity column (Macherey-Nagel, Allentown, PA). Mobile phases comprised of 1% 1 M ammonium acetate, 0.1 % acetic acid in water (A) and 1% 1 M ammonium acetate, 0.1% acetic acid in 7:3 (v/v) acetonitrile: isopropanol (B). The following gradient modified from a previously described method27 to adapt to micro flow was used; 0-1 min at 55% B, 4 min at 75% B, 12 min at 89% B, 15 min at 99% B, 18 min at 99% B and 20 min at 55% B followed by equilibration up to 30 min.
Instrument Name:Dionex UltiMate 3000 RSLCnano
Column Name:Custom C8 - Higgins Analytical
Column Temperature:40
Flow Rate:0.04 mL min-1
Internal Standard:Equisplash
Solvent A:100% water; 0.1% acetic acid; 10 mM ammonium acetate
Solvent B:70% acetonitrile/30% isopropanol; 0.1% acetic acid; 10 mM ammonium acetate
Chromatography Type:Reversed phase
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