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MB Sample ID: SA214085
Local Sample ID: | 8_WT_4-neg |
Subject ID: | SU002326 |
Subject Type: | Plant |
Subject Species: | Arabidopsis thaliana |
Taxonomy ID: | 3702 |
Age Or Age Range: | 10 day old seedlings |
Species Group: | Roots |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003657 | AN003658 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Intensity | Intensity |
Chromatography:
Chromatography ID: | CH002710 |
Chromatography Summary: | Chromatographic separation using HILIC was achieved using an Agilent 1290 Infinity II UHPLC system equipped with a SeQuant® ZIC®-HILIC (100 x 2.1 x 3.5 µm) column (EMD Millipore, Burlington, MA). Mobile phases A and B were comprised of 5 mM ammonium acetate (pH 4.0) in water and 90% acetonitrile with 0.1 % acetic acid, respectively. A flow rate of 0.3 mL min-1 was used to elute compounds with the following gradient: 87% B for 5 minutes, decreased to 55% B over the next 8 minutes and held for 2.5 minutes before returning to 87% and equilibrating the column for 3 minutes. |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
Column Temperature: | 40 |
Flow Rate: | 0.3 mL min-1 |
Internal Standard: | Equisplash |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 4 |
Solvent B: | 90% acetonitrile/10% water; 0.1% acetic acid |
Chromatography Type: | HILIC |