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MB Sample ID: SA217814
| Local Sample ID: | A03-01_59_1_1701 |
| Subject ID: | SU002359 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Not applicable |
| Cell Biosource Or Supplier: | Sigma-Aldrich (St. Louis, MO) |
| Cell Strain Details: | Hep3B |
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Combined analysis:
| Analysis ID | AN003715 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | UHPLC-QTOF-MS |
| Column | Hamilton Intensity Solo 2 C18 (100 mm x 2.1 mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Trapped Ion Mobility Q-TOF |
| MS instrument name | Bruker timsTOF |
| Ion Mode | POSITIVE |
| Units | AU |
Chromatography:
| Chromatography ID: | CH002752 |
| Chromatography Summary: | The Elute UHPLC and Q-TOF Mass Spectrometer (Bruker, Bremen, Germany) were utilized for metabolite and peptide detection. The Elute HPG 1300 pumps, Elute Au-tosampler (Bruker, Bremen, Germany), and Hamilton® Intensity Solo 2 C18 column (100 mm x 2.1 mm, 1.8 m beads) were employed using reversed-phase chromatog-raphy. Solvents used for separation were 0.1 % FA in LC grade water (solvent A) and 0.1 % FA in ACN (solvent B). Each metabolite and protein extract were analyzed in duplicate. For metabolomics analysis, the column was kept at 35°C, and each sample was injected twice with an in-jection volume of 10 µL. Sample elution was performed in 30 min gradient starting with 1% ACN for 2 min and then ramped to 99% ACN within 15 min. After that, 99% ACN was kept for 3 min, and then the re-equilibration to 1% ACN was done for 10 min. The flow rate was 0.25 mL/min for 20 min and then 0.35 mL/min for 8.3 min and then the flow rate set at 0.25 mL/min for 1.7 min. |
| Instrument Name: | UHPLC-QTOF-MS |
| Column Name: | Hamilton Intensity Solo 2 C18 (100 mm x 2.1 mm,1.8um) |
| Column Temperature: | 35°C |
| Flow Gradient: | Sample elution was performed in 30 min gradient starting with 1% ACN for 2 min and then ramped to 99% ACN within 15 min. After that, 99% ACN was kept for 3 min, and then the re-equilibration to 1% ACN was done for 10 min |
| Flow Rate: | was 0.25 mL/min for 20 min and then 0.35 mL/min for 8.3 min and then the flow rate set at 0.25 mL/min for 1.7 min. |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
