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MB Sample ID: SA217829
| Local Sample ID: | SPT_C133W_1h_01311114 |
| Subject ID: | SU002360 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC, CCL-247 |
| Cell Strain Details: | HCT116 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003716 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Kinetex C8 (150 x 3mm,2.6um,100Å) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | ion counts |
Chromatography:
| Chromatography ID: | CH002753 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Kinetex C8 (150 x 3mm,2.6um,100Å) |
| Column Temperature: | 40 |
| Flow Gradient: | The LC gradient held at 82% B for 0-3 min, then ran from 82%-90% B in 3-4 min, then 90-99% in 4-18 min, held at 99% B for 7 min, then reduced from 99%-82% from 25-27 min, then held at 82% for a further 13 mins. |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 100% water; 0.2% formic acid; 2 mM ammonium formate |
| Solvent B: | 100% methanol; 0.2% formic acid; 1 mM ammonium formate |
| Chromatography Type: | Reversed phase |
