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MB Sample ID: SA217835
Local Sample ID: | SPT_WT_15min_0131111 |
Subject ID: | SU002360 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | ATCC, CCL-247 |
Cell Strain Details: | HCT116 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003716 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Kinetex C8 (150 x 3mm,2.6um,100Å) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | ion counts |
Chromatography:
Chromatography ID: | CH002753 |
Instrument Name: | Thermo Vanquish |
Column Name: | Kinetex C8 (150 x 3mm,2.6um,100Å) |
Column Temperature: | 40 |
Flow Gradient: | The LC gradient held at 82% B for 0-3 min, then ran from 82%-90% B in 3-4 min, then 90-99% in 4-18 min, held at 99% B for 7 min, then reduced from 99%-82% from 25-27 min, then held at 82% for a further 13 mins. |
Flow Rate: | 0.5 mL/min |
Solvent A: | 100% water; 0.2% formic acid; 2 mM ammonium formate |
Solvent B: | 100% methanol; 0.2% formic acid; 1 mM ammonium formate |
Chromatography Type: | Reversed phase |